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HighYield T7 AF647 RNA Labeling Kit
| 貨號 | RNT-101-AF647 | 售價（元） | 5475 |
| 規(guī)格 | 20T x 20 μl | CAS號 |
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產(chǎn)品信息
|
品牌 |
貨號 |
名稱 |
規(guī)格 |
單價 |
|
Jena Bioscience |
RNT-101-AF647 |
HighYield T7 AF647 RNA Labeling Kit |
20T x 20 μl |
5475.00 |
HighYield T7 AF647 RNA標(biāo)記試劑盒旨在通過體外轉(zhuǎn)錄產(chǎn)生隨機的AF647修飾RNA探針。這種探針非常適合原位雜交和Northern Blot實驗。AF647（Alexa Fluor?647的結(jié)構(gòu)類似物）是一種親水性染料，與Cy5相比，其光穩(wěn)定性更強。
UTP-PEG5-AF647使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標(biāo)記聚合酶混合物有效地并入RNA中，作為天然對應(yīng)物UTP的替代物。35%UTP-PEG5-AF647替代通常會在反應(yīng)和標(biāo)記效率之間實現(xiàn)最佳平衡。然而，UTP-PEG5-AF647/UTP比值的單獨優(yōu)化可以通過單核苷酸格式輕松實現(xiàn)。由此產(chǎn)生的AF647修飾RNA探針隨后可以通過熒光光譜檢測。
該試劑盒含有足夠的試劑，用于20個標(biāo)記反應(yīng)，每個反應(yīng)20μl（35%UTP-PEG5-AF647替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM UTP-PEG-5-AF646）。20μl反應(yīng)在30分鐘培養(yǎng)后產(chǎn)生約4-6μg RNA（1μg T7對照模板，1.4 kb RNA轉(zhuǎn)錄物）。然而，產(chǎn)量可能因模板而異（啟動子設(shè)計、序列長度、二級結(jié)構(gòu)形成）。
Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
UTP-PEG5-AF647
1x 10 μl (5 mM)
T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)
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|
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20reactions x 20 μl |
4875.00 |
|
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20reactions x 20 μl |
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30reactions x 20 μl |
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|
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25reactions x 20 μl |
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HighYield T7 AF647 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
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20reactions x 20 μl |
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20reactions x 20 μl |
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