HighYield T7 Azide RNA Labeling Kit_南京沃博生物科技有限公司

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HighYield T7 Azide RNA Labeling Kit

貨號	RNT-101-AZ	售價（元）	3975
規(guī)格	25T	CAS號

產(chǎn)品簡介
相關(guān)產(chǎn)品

產(chǎn)品信息

品牌	貨號	名稱	規(guī)格	單價
Jena Bioscience	RNT-101-AZ	HighYield T7 Azide RNA Labeling Kit	25T x 20 μl	3975.00

產(chǎn)品簡介

HighYield T7疊氮RNA標記試劑盒旨在通過體外轉(zhuǎn)錄產(chǎn)生隨機疊氮修飾的RNA探針。這種探針非常適合原位雜交、Northern Blot或交聯(lián)實驗。

使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標記聚合酶混合物，5-疊氮-C3-UTP有效地并入RNA中，作為其天然對應(yīng)物UTP的替代物。35%5-疊氮-C3-UTP取代通常會在反應(yīng)和標記效率之間實現(xiàn)最佳平衡。然而，5-Azido-C3-UTP/UTP比值的單獨優(yōu)化可以通過單核苷酸格式輕松實現(xiàn)。由此產(chǎn)生的疊氮化物修飾RNA探針隨后可通過無銅點擊化學用二苯并環(huán)辛炔（DBCO）功能化檢測試劑（例如DBCO功能化熒光染料或（Desthio）生物素）標記，從而形成穩(wěn)定的共價標記RNA探針[1]。該試劑盒含有足夠的試劑，用于25個標記反應(yīng)，每個反應(yīng)20μl（35%5-疊氮-C3-UTP取代，1 mM ATP，GTP，CTP，0.65 mM UTP，0.35 mM 5-疊氮-C3-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

5-Azido-C3-UTP
1x 20 μl (10 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
DBCO-functionalized detection molecule
RNA purification tools
RNAse-free DNAse I (optional)

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HighYield T7 Azide RNA Labeling Kit