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HighYield T7 AF594 RNA Labeling Kit

貨號 RNT-101-AF594 售價(元) 5475
規(guī)格 20T x 20 μl CAS號
  • 產(chǎn)品簡介
  • 相關(guān)產(chǎn)品

產(chǎn)品信息

品牌

貨號

名稱

規(guī)格

單價

Jena Bioscience

RNT-101-AF594

HighYield T7 AF594 RNA Labeling Kit

20T x 20 μl

5475.00

產(chǎn)品簡介

      HighYield T7 AF594 RNA標記試劑盒設(shè)計用于通過體外轉(zhuǎn)錄隨機產(chǎn)生AF594修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實驗。AF594(也稱為Alexa Fluor?594)是一種親水性染料,與德克薩斯紅相比,其光穩(wěn)定性更強。

      UTP-PEG5-AF594使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標記聚合酶混合物有效地并入RNA中,作為其天然對應(yīng)物UTP的替代物。35%UTP-PEG5-AF594替代通常會在反應(yīng)和標記效率之間實現(xiàn)最佳平衡。然而,UTP-PEG5-AF594/UTP比值的單獨優(yōu)化可以通過單核苷酸格式輕松實現(xiàn)。由此產(chǎn)生的AF594修飾RNA探針隨后可以通過熒光光譜檢測。

      該試劑盒含有足夠的試劑,用于20個標記反應(yīng),每個反應(yīng)20μl35%UTP-PEG5-AF594替代物、2.5 mM ATP、GTPCTP、0.2 mM UTP、0.1 mM UTP-PEG-5-AF595)。培養(yǎng)30分鐘后,20μl反應(yīng)產(chǎn)生約5-7μg RNA1μg T7對照模板,1.4 kb RNA轉(zhuǎn)錄物)。然而,產(chǎn)量可能因模板而異(啟動子設(shè)計、序列長度、二級結(jié)構(gòu)形成)。


Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

UTP-PEG5-AF594
1x 10 μl (5 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

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