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HighYield T7 AF594 RNA Labeling Kit
貨號 | RNT-101-AF594 | 售價(元) | 5475 |
規(guī)格 | 20T x 20 μl | CAS號 |
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產(chǎn)品信息
品牌 |
貨號 |
名稱 |
規(guī)格 |
單價 |
Jena Bioscience |
RNT-101-AF594 |
HighYield T7 AF594 RNA Labeling Kit |
20T x 20 μl |
5475.00 |
HighYield T7 AF594 RNA標記試劑盒設(shè)計用于通過體外轉(zhuǎn)錄隨機產(chǎn)生AF594修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實驗。AF594(也稱為Alexa Fluor?594)是一種親水性染料,與德克薩斯紅相比,其光穩(wěn)定性更強。
UTP-PEG5-AF594使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標記聚合酶混合物有效地并入RNA中,作為其天然對應(yīng)物UTP的替代物。35%UTP-PEG5-AF594替代通常會在反應(yīng)和標記效率之間實現(xiàn)最佳平衡。然而,UTP-PEG5-AF594/UTP比值的單獨優(yōu)化可以通過單核苷酸格式輕松實現(xiàn)。由此產(chǎn)生的AF594修飾RNA探針隨后可以通過熒光光譜檢測。
該試劑盒含有足夠的試劑,用于20個標記反應(yīng),每個反應(yīng)20μl(35%UTP-PEG5-AF594替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM UTP-PEG-5-AF595)。培養(yǎng)30分鐘后,20μl反應(yīng)產(chǎn)生約5-7μg RNA(1μg T7對照模板,1.4 kb RNA轉(zhuǎn)錄物)。然而,產(chǎn)量可能因模板而異(啟動子設(shè)計、序列長度、二級結(jié)構(gòu)形成)。
Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
UTP-PEG5-AF594
1x 10 μl (5 mM)
T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)
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50reactions x 20 μl |
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20reactions x 20 μl |
4875.00 |
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HighYield T7 Digoxigenin RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
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RNT-101-CY5 |
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20reactions x 20 μl |
4875.00 |
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20reactions x 20 μl |
4875.00 |
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30reactions x 20 μl |
4425.00 |
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30reactions x 20 μl |
4425.00 |
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25reactions x 20 μl |
3975.00 |
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HighYield T7 AF647 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
Jena Bioscience |
RNT-101-AF594 |
HighYield T7 AF594 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
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RNT-101-AF555 |
HighYield T7 AF555 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
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20reactions x 20 μl |
5475.00 |
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20reactions x 20 μl |
5925.00 |
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HighYield T7 Atto488 RNA Labeling Kit |
50reactions x 20 μl |
13755.00 |
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HighYield T7 RNA Synthesis Kit |
50reactions x 20 μl |
2925.00 |