HighYield T7 AF555 RNA Labeling Kit_南京沃博生物科技有限公司

熱銷產(chǎn)品

團(tuán)購(gòu)專區(qū)

試用專區(qū)

當(dāng)前位置：首頁(yè) - 產(chǎn)品專區(qū) - 熱銷產(chǎn)品

HighYield T7 AF555 RNA Labeling Kit

貨號(hào)	RNT-101-AF555	售價(jià)（元）	5475
規(guī)格	20T x 20 μl	CAS號(hào)

產(chǎn)品簡(jiǎn)介
相關(guān)產(chǎn)品

產(chǎn)品信息

品牌	貨號(hào)	名稱	規(guī)格	單價(jià)
Jena Bioscience	RNT-101-AF555	HighYield T7 AF555 RNA Labeling Kit	20T x 20 μl	5475.00

產(chǎn)品簡(jiǎn)介

HighYield T7 AF555 RNA標(biāo)記試劑盒設(shè)計(jì)用于通過(guò)體外轉(zhuǎn)錄隨機(jī)產(chǎn)生AF555修飾的RNA探針。這種探針?lè)浅＿m合原位雜交和Northern Blot實(shí)驗(yàn)。AF55（Alexa Fluor?555的結(jié)構(gòu)類似物）是一種親水性染料，與Cy3相比，其光穩(wěn)定性更強(qiáng)。

UTP-PEG5-AF555使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標(biāo)記聚合酶混合物有效地并入RNA中，作為天然對(duì)應(yīng)物UTP的替代物。35%UTP-PEG5-AF555替代通常會(huì)在反應(yīng)和標(biāo)記效率之間實(shí)現(xiàn)最佳平衡。然而，UTP-PEG5-AF555/UTP比值的單獨(dú)優(yōu)化可以通過(guò)單核苷酸格式輕松實(shí)現(xiàn)。由此產(chǎn)生的AF555修飾RNA探針隨后可以通過(guò)熒光光譜檢測(cè)。

該試劑盒含有足夠的試劑，用于20個(gè)標(biāo)記反應(yīng)，每個(gè)反應(yīng)20μl（35%UTP-PEG5-AF555替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM UTP-PEG-5-AF552）。培養(yǎng)30分鐘后，20μl反應(yīng)產(chǎn)生約5-7μg RNA（1μg T7對(duì)照模板，1.4 kb RNA轉(zhuǎn)錄物）。然而，產(chǎn)量可能因模板而異（啟動(dòng)子設(shè)計(jì)、序列長(zhǎng)度、二級(jí)結(jié)構(gòu)形成）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

UTP-PEG5-AF555
1x 10 μl (5 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

相關(guān)產(chǎn)品

品牌	貨號(hào)	名稱	規(guī)格	單價(jià)
Jena Bioscience	RNT-106-S	HighYield T7 mRNA Synthesis Kit (Ψ-UTP)	15reactions x 20 μl	4425.00
Jena Bioscience	RNT-106-L	HighYield T7 mRNA Synthesis Kit (Ψ-UTP)	50reactions x 20 μl	8925.00
Jena Bioscience	RNT-105	HighYield T7 sgRNA Synthesis Kit (SpCas9)	50reactions	4425.00
Jena Bioscience	RNT-104	HighYield T7 mRNA Synthesis Kit (m5CTP/Ψ-UTP)	15reactions x 20 μl	7875.00
Jena Bioscience	RNT-103	HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/Ψ-UTP)	15reactions x 20 μl	8805.00
Jena Bioscience	RNT-102-S	HighYield T7 ARCA mRNA Synthesis Kit	15reactions x 20 μl	4125.00
Jena Bioscience	RNT-102-L	HighYield T7 ARCA mRNA Synthesis Kit	50reactions x 20 μl	8175.00
Jena Bioscience	RNT-101-FAMX	HighYield T7 Fluorescein RNA Labeling Kit	20reactions x 20 μl	4875.00
Jena Bioscience	RNT-101-DIGX	HighYield T7 Digoxigenin RNA Labeling Kit	30reactions x 20 μl	4425.00
Jena Bioscience	RNT-101-CY5	HighYield T7 Cy5 RNA Labeling Kit	20reactions x 20 μl	4875.00
Jena Bioscience	RNT-101-CY3	HighYield T7 Cy3 RNA Labeling Kit	20reactions x 20 μl	4875.00
Jena Bioscience	RNT-101-BIOX	HighYield T7 Biotin11 RNA Labeling Kit	30reactions x 20 μl	4425.00
Jena Bioscience	RNT-101-BIO16	HighYield T7 Biotin16 RNA Labeling Kit	30reactions x 20 μl	4425.00
Jena Bioscience	RNT-101-AZ	HighYield T7 Azide RNA Labeling Kit	25reactions x 20 μl	3975.00
Jena Bioscience	RNT-101-AF647	HighYield T7 AF647 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-AF594	HighYield T7 AF594 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-AF555	HighYield T7 AF555 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-AF488	HighYield T7 AF488 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-488-S	HighYield T7 Atto488 RNA Labeling Kit	20reactions x 20 μl	5925.00
Jena Bioscience	RNT-101-488-L	HighYield T7 Atto488 RNA Labeling Kit	50reactions x 20 μl	13755.00
Jena Bioscience	RNT-101	HighYield T7 RNA Synthesis Kit	50reactions x 20 μl	2925.00

上一頁(yè)： HighYield T7 AF594 RNA Labeling Kit
下一頁(yè)： HighYield T7 AF488 RNA Labeling Kit

天天操夜夜操,一超碰,亚洲AV片在线观看,亚洲骚国产精品中文

HighYield T7 AF555 RNA Labeling Kit