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HighYield T7 AF488 RNA Labeling Kit
| 貨號(hào) | RNT-101-AF488 | 售價(jià)（元） | 5475 |
| 規(guī)格 | 20T x 20 μl | CAS號(hào) |
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產(chǎn)品信息
|
品牌 |
貨號(hào) |
名稱 |
規(guī)格 |
單價(jià) |
|
Jena Bioscience |
20reactions x 20 μl |
5475.00 |
HighYield T7 AF488 RNA標(biāo)記試劑盒設(shè)計(jì)用于通過體外轉(zhuǎn)錄隨機(jī)產(chǎn)生AF488修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實(shí)驗(yàn)。AF488（也稱為Alexa Fluor?488）是一種親水性染料，與熒光素相比，其光穩(wěn)定性更強(qiáng)。
UTP-PEG5-AF488使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標(biāo)記聚合酶混合物有效地并入RNA中，作為天然對(duì)應(yīng)物UTP的替代物。35%UTP-PEG5-AF488替代通常會(huì)在反應(yīng)和標(biāo)記效率之間實(shí)現(xiàn)最佳平衡。然而，UTP-PEG5-AF488/UTP比值的單獨(dú)優(yōu)化可以通過單核苷酸格式輕松實(shí)現(xiàn)。由此產(chǎn)生的AF488修飾RNA探針隨后可以通過熒光光譜檢測(cè)。
該試劑盒含有足夠的試劑，用于20個(gè)標(biāo)記反應(yīng)，每個(gè)反應(yīng)20μl（35%UTP-PEG5-AF488替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM UTP-PEG-5-AF488.）。
培養(yǎng)30分鐘后，20μl反應(yīng)產(chǎn)生約5-7μg RNA（1μg T7對(duì)照模板，1.4 kb RNA轉(zhuǎn)錄物）。然而，產(chǎn)量可能因模板而異（啟動(dòng)子設(shè)計(jì)、序列長(zhǎng)度、二級(jí)結(jié)構(gòu)形成）。
Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
UTP-PEG5-AF488
1x 10 μl (5 mM)
T7 G-initiating control template (1.4
kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor
resulting in approx. 1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)
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貨號(hào) |
名稱 |
規(guī)格 |
單價(jià) |
|
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30reactions x 20 μl |
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20reactions x 20 μl |
4875.00 |
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20reactions x 20 μl |
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30reactions x 20 μl |
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30reactions x 20 μl |
4425.00 |
|
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25reactions x 20 μl |
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5475.00 |
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RNT-101-AF594 |
HighYield T7 AF594 RNA Labeling Kit |
20reactions x 20 μl |
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HighYield T7 AF555 RNA Labeling Kit |
20reactions x 20 μl |
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RNT-101-AF488 |
HighYield T7 AF488 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
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20reactions x 20 μl |
5925.00 |
|
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50reactions x 20 μl |
13755.00 |
|
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50reactions x 20 μl |
2925.00 |