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ChromoTek GFP-Trap? Agarose
| 貨號 | gta | 售價（元） | 4350 |
| 規(guī)格 | 20rxns | CAS號 |
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Product Information
GFP-Trap? Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
| Description | Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.
? Fast, reliable & efficient one-step immunoprecipitation ? Ready-to-use ? No heavy & light antibody chains ? Stable under harsh washing conditions ? Suitable for downstream mass spec analysis ? Works in samples from: mammals, plants, bacteria, yeast, insects etc. |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP For the complete list, please click here:?Fluorescent protein specificity table |
| Binding capacity | 25-30 μg of recombinant GFP per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 1 mM DTT, 3 M Guanidinium?HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD?of 1 pM |
| Compatibility with mass spectrometry | The GFP-Trap? is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631357 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |