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產(chǎn)品信息

     Plasmid-Safe? ATP-Dependent DNase selectively hydrolyzes linear double-stranded (ds) DNA to deoxynucleotides at slightly alkaline pH and, with a lower efficiency, linear and closed-circular   single-stranded DNAs. The reaction is ATP-dependent, and does not affect   closed-circular supercoiled or nicked circular dsDNAs. The enzyme can be conveniently and completely heat-inactivated by a 30 minute incubation at   70°C. Plasmid-Safe DNase is useful as a final “cleanup” of DNA preparations  from plasmid and cosmid clones, to avoid the problems caused by contaminating  genomic DNA.
     粘粒、BAC、fosmid和質(zhì)粒制備物中去除污染的細菌染色體DNA。該酶將從末端逐步降解線性DNA；閉環(huán)DNA(例如質(zhì)粒)不具有游離末端，因此不會被降解。這些特性使得Plasmid-Safe ATP-Dependent DNase成為BAC和fosmid純化方案的理想選擇，例如用于鳥槍測序，以及其他需要高純度DNA的應用。
優(yōu)點：
- Digest contaminating linear DNA without digesting nicked or closed-circular dsDNA or     supercoiled DNA (plasmids, fosmids, etc.)；
- Use as a final purification step for plasmid, cosmid,  fosmid, and BAC vector and clone     preparations
- Utilize whenever protecting circular dsDNA is important
- 消化污染的線性DNA，而不消化有缺口或封閉的圓形dsDNA或超螺旋DNA（質(zhì)粒，粘粒等）；
- 用作質(zhì)粒，cosmid, fosmid，BAC載體和克隆制劑的最終純化步驟；
- 在保護重要環(huán)狀dsDNA時使用.

組成成分：
? Plasmid-Safe?  ATPDependent DNase （10U/μL）：1mL； -20°C
? Plasmid-Safe? 10X   Buffer：5mL；-20°C
? ATP Solution (25 mM)：2m L； -20°C