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產(chǎn)品信息

      EpiScript? RNase H– Reverse Transcriptase (EpiScript RT), an alternative to SuperScript? II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity. It is  highly efficient at producing full-length cDNA from long RNA templates.  EpiScript RT demonstrates significantly more activity than other MMLV Reverse transcriptase enzymes. It is capable of producing cDNA from as little as 100pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.  
 EpiScript RT is supplied at a concentration of 200U/μL. A 10X RT Reaction Buffer and 100 mM dithiothreitol (DTT) are also provided.
 EpiScript?RNase H –逆轉(zhuǎn)錄酶（EpiScript RT）是SuperScript?II逆轉(zhuǎn)錄酶的替代品，是一種重組MMLV逆轉(zhuǎn)錄酶，其RNase H活性降低。 從長(zhǎng)RNA模板產(chǎn)生全長(zhǎng)cDNA的效率很高。EpiScript RT具有比其他MMLV逆轉(zhuǎn)錄酶更高的活性。它能夠從少至100pg的總RNA中產(chǎn)生cDNA，用于實(shí)時(shí)RT-PCR（qRT-PCR）分析和其他應(yīng)用。
 EpiScript RT的濃度為200U /μL。還提供了10X RT反應(yīng)緩沖液和100 mM二硫蘇糖醇（DTT）。
 EpiScript Reverse Trascriptase可以非常高效地從RNA模板產(chǎn)生長(zhǎng)度達(dá)12kb的全長(zhǎng)cDNA。該酶經(jīng)基因工程改造以顯著降低RNase H活性。這種結(jié)構(gòu)修飾消除了第一鏈cDNA合成過(guò)程中RNA分子的降解，使得EpiScrit Reverse Transcriptase在實(shí)時(shí)RT-PCR分析和其他應(yīng)用中表現(xiàn)出卓越的性能。
優(yōu)點(diǎn)：
? Produce full-length cDNA with RNAse H- mutant；
? Achieve higher specificity at elevated temperatures, up to 55°C
組成成分：
? EpiScript RNase H- Reverse Transcriptase（200U/μL）：125μL； -20°C
? 10X Reaction Buffer：600μL；-20°C
? DTT（100 mM）：600μL；-20°C