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HighYield T7 Fluorescein RNA Labeling Kit

貨號	RNT-101-FAMX	售價（元）	4875
規(guī)格	20Tx 20 μl	CAS號

產(chǎn)品簡介
相關產(chǎn)品

產(chǎn)品信息

品牌	貨號	名稱	規(guī)格	單價
Jena Bioscience	RNT-101-FAMX	HighYield T7 Fluorescein RNA Labeling Kit	20Tx 20 μl	4875.00

產(chǎn)品簡介

HighYield T7熒光素RNA標記試劑盒設計用于通過體外轉錄產(chǎn)生隨機熒光素修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實驗。標記原理類似于熒光素RNA標記混合物（羅氏）的基本標記原理。熒光素-12-UTP通過優(yōu)化反應緩沖液和T7 RNA標記聚合酶混合物有效地并入RNA中，作為其天然對應物UTP的替代物。35%熒光素-12-UTP替代通常會在反應和標記效率之間實現(xiàn)最佳平衡。然而，熒光素-12-UTP/UTP比值的個體優(yōu)化可以通過單核苷酸格式輕松實現(xiàn)。由此產(chǎn)生的熒光素修飾RNA探針隨后可通過熒光光譜檢測。

試劑盒包含足夠的試劑，用于20個標記反應，每個反應20μl（35%熒光素-12-UTP替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM熒光素12-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

Fluorescein-12-UTP
1x 10 μl (5 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

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HighYield T7 Fluorescein RNA Labeling Kit