HighYield T7 Digoxigenin RNA Labeling Kit_南京沃博生物科技有限公司

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HighYield T7 Digoxigenin RNA Labeling Kit

貨號(hào)	RNT-101-DIGX	售價(jià)（元）	4425
規(guī)格	30T x 20 μl	CAS號(hào)

產(chǎn)品簡介
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產(chǎn)品信息

品牌	貨號(hào)	名稱	規(guī)格	單價(jià)
Jena Bioscience	RNT-101-DIGX	HighYield T7 Digoxigenin RNA Labeling Kit	30T x 20 μl	4425.00

產(chǎn)品簡介

HighYield T7地高辛RNA標(biāo)記試劑盒設(shè)計(jì)用于通過體外轉(zhuǎn)錄隨機(jī)產(chǎn)生地高辛修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實(shí)驗(yàn)。標(biāo)記原則類似于DIG RNA標(biāo)記混合物（羅氏）和DIG RNA標(biāo)簽試劑盒（羅氏公司）的基本標(biāo)記原則。使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標(biāo)記聚合酶混合物，DIG-11-UTP作為天然對應(yīng)物UTP的替代物有效地并入RNA。35%DIG-11-UTP替代通常會(huì)在反應(yīng)和標(biāo)記效率之間實(shí)現(xiàn)最佳平衡。然而，使用單核苷酸格式可以輕松實(shí)現(xiàn)DIG-11-UTP/UTP比值的個(gè)別優(yōu)化。由此產(chǎn)生的地高辛修飾RNA探針隨后可通過HRP或AP修飾地高辛抗體檢測。

該試劑盒含有足夠的試劑，用于30個(gè)標(biāo)記反應(yīng)，每個(gè)反應(yīng)20μl（35%DIG-11-UTP替代物，1 mM ATP，GTP，CTP，0.65 mM UTP，0.35 mM DIG-11-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

DIG-11-UTP
1x 25 μl (10 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

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HighYield T7 Digoxigenin RNA Labeling Kit