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pIMAGO HRP Phosphoprotein Detection on Western Blot (complete kit)

貨號(hào) 800-40 售價(jià)(元) 5500
規(guī)格 1 kit CAS號(hào)
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產(chǎn)品簡(jiǎn)介:

? ? ? ? 磷酸化在許多細(xì)胞功能的調(diào)節(jié)中起著關(guān)鍵作用。常見(jiàn)的磷酸化檢測(cè)方法包括昂貴且通常不可用的磷酸化抗體或不安全的放射性ATP標(biāo)記。我們提供pIMAGO?C是一種新型、非抗體、高效且安全的產(chǎn)品,可無(wú)偏見(jiàn)地普遍檢測(cè)任何磷蛋白。與抗體不同,pIMAGO能夠獨(dú)立于氨基酸序列選擇性結(jié)合到磷酸化殘基。該pIMAGO試劑盒能夠使用化學(xué)發(fā)光(HRP/過(guò)氧化物酶)檢測(cè)Western Blot格式(膜上)的磷酸蛋白。該試劑盒包括pIMAGO生物素試劑、抗生物素HRP(抗生物素過(guò)氧化物酶)檢測(cè)試劑、阻斷緩沖液、pIMAGO緩沖液、洗滌緩沖液和對(duì)照磷酸蛋白。不包括ECL基板。?


? ? ? ? Phosphorylation plays a critical role in the regulation of many cellular functions. Common phosphorylation detection methods include expensive and often unavailable phospho-antibodies or unsafe radioactive ATP labeling. We offer pIMAGO – a novel, non-antibody, highly effective and safe product for unbiased universal detection of any phosphoprotein. Unlike antibodies, pIMAGO is capable of selective binding to a phosphorylated residue independent of amino acid sequence. This pIMAGO kit enables the detection of phosphoproteins in Western Blot format (on-membrane) using chemiluminescence (HRP/peroxidase). The kit includes pIMAGO-biotin reagent, avidin-HRP (avidin-peroxidase) detection reagent, blocking buffer, pIMAGO buffer, washing buffer and a control phosphoprotein. ECL substrate is not included.