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產(chǎn)品信息

產(chǎn)品性質(zhì)

品牌 Spirochrome

λabs 480 nm

λfl 600 nm

εmax 1.66·104 mol-1·cm-1 (in DMSO)

lifetime 2.8-7 ns

QY 30% (in AcOEt)

運(yùn)輸 常溫

儲(chǔ)存 -20℃

產(chǎn)品簡(jiǎn)介

Lyso Flipper-TR? is a lysosome specific live cell fluorescent membrane tension probe. It is the first commercially available lysosome specific fluorescent membrane tension reporter tailored to the field of mechanobiology. Take advantage of the vast and new field Lyso Flipper-TR? gives access to and test it on your favourite mechanobiological project.

Contains 35 nmol Lyso Flipper-TR? probe. Probe quantity allows 35 – 150 staining experiments.*

Lyso Flipper-TR? is a fluorescent probe that specifically targets the membrane of lysosomes of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains a lysosome targeting motive as welll as a Flipper fluorophore which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Lyso Flipper-TR?  spontaneously labels lysosomes of cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. A detailed protocol for staining and  FLIM imaging can be found in the datasheet. Probe quantity allows 35-150 staining experiments.*

*Based on the following conditions: 0.5-1 ml staining solution / staining experiment with 0.5-1uM probe concentration. The number of staining experiments can be further increased by reducing  volume or the probe concentration.

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