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Expresso? T7 SUMO Cloning and Expression System，Expresso? T7 SUMO克隆及蛋白表達(dá)系統(tǒng)
| 貨號(hào) | 49003-1/2 | 售價(jià)（元） | 3999/7208 |
| 規(guī)格 | 5T/10T | CAS號(hào) |
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產(chǎn)品信息
|
貨號(hào) |
名稱(chēng) |
規(guī)格 |
價(jià)格/元 |
|
49060-1 |
Expresso? Solubility and Express Screening System/Expresso?溶解度和表達(dá)篩選系統(tǒng) |
24T |
11152 |
|
49062-1 |
Expresso? Solubility and Expression Screening System + SelecTEV? Protease |
24T |
14972 |
|
49064-1 |
Expresso? Solubility and Expression Screening System + Accura? High-Fidelity Polymerase，Expresso?溶解度和表達(dá)篩選系統(tǒng)+Accura?高保真聚合酶 |
24T |
13098 |
|
49066-1 |
Expresso? Solubility and Expression Screening System + SelecTEV? Protease + Accura? High-Fidelity Polymerase，Expresso?溶解度和表達(dá)篩選系統(tǒng)+ EV?蛋白酶+Accura?高保真聚合酶 |
24T |
15404 |
|
49001-1/2 |
Expresso? T7 Cloning and Expression System, N-His Expresso? T7克隆和蛋白表達(dá)系統(tǒng) |
5T/10T |
2901/5320 |
|
49002-1/2 |
Expresso? T7 Cloning and Expression System, C-His，Expresso? T7克隆和蛋白表達(dá)系統(tǒng) |
5T/10T |
2901/5271 |
|
49003-1/2 |
Expresso? T7 SUMO Cloning and Expression System，Expresso? T7 SUMO克隆及蛋白表達(dá)系統(tǒng) |
5T/10T |
3999/7208 |
|
49011-1/2 |
Expresso? Rhamnose Cloning and Expression Syst.,N-His/Expresso? Rhamnose克隆及蛋白表達(dá)系統(tǒng) |
5T/10T |
2901/5320 |
|
49012-1/2 |
Expresso? Rhamnose Cloning and Expression System, C-His，Expresso? Rhamnose克隆及蛋白表達(dá)系統(tǒng) |
5T/10T |
2901/5320 |
|
49013-1/2 |
Expresso? Rhamnose SUMO Cloning and Expression System，Expresso? Rhamnose SUMO克隆及蛋白表達(dá)系統(tǒng) |
5T/10T |
4036/7208 |
The Expresso SUMO Cloning and Protein Expression Systems are designed for fast, easy, and efficient directional cloning and soluble expression of PCR-amplified genes. For proteins that form inclusion bodies or are difficult to express in soluble form, the pETite? N-His SUMO and pRham? N-His SUMO Vectors allow expression of target proteins with an amino-terminal 6xHis-SUMO protein tag. SUMO (small ubiquitin-like modifier) is a relatively small (100-residue) polypeptide that has been shown to enhance the soluble expression of many proteins that are otherwise difficult to produce in E. coli.
優(yōu)點(diǎn)：
? Enzyme-free directional PCR cloning in seconds!
? Save days of effort with ready-to-use vectors and competent cells - NO ligation step.
? Tightly-controlled expression of N-terminal 6xHis-tagged proteins with cleavable SUMO solubility tag.
數(shù)據(jù)
Figure 1. SUMO expression vector: Small size (2.5 kb vs. 5.4 kb for pET) for easier manipulation, including targeted mutagenesis. Expression plasmid is pre-processed for instant enzyme-free cloning of PCR products.
Figure 2. Large-scale cloning and expression case study: (A) PCR products from 48 putative hydrolase genes ranging from ~1 to >3 kb. (B) Uninduced (-) and IPTG-induced (+) samples of HI-Control BL21(DE3) cells with 6 different genes cloned into the pETite C-His Vector. (C) Enhanced solubility of SUMO-tagged 2201 and 2442 gene products. Total cell extract and soluble fractions are shown. (D) Removal of 6xHis-SUMO tag from purified SUMO-2201 fusion protein by SUMO protease. –prot: uncleaved SUMO-2201 fusion protein after IMAC purification; +prot: SUMO protease-treated fusion protein; C: isolated 2201 protein after removal of 6xHis-SUMO fragment and SUMO protease by subtractive IMAC.
相關(guān)產(chǎn)品：
? Expresso? T7 Cloning and Expression System, N-His（貨號(hào)#49001）
? Expresso? T7 Cloning and Expression System, C-His（貨號(hào)#49002）
? HI-Control? 10G Chemically Competent Cells (SOLOs)（貨號(hào)#60110-1）
? HI-Control? BL21(DE3) Chemically Competent Cells (SOLOs)（貨號(hào)#60435-1）
? SUMO Express Protease（貨號(hào)#30801）